/
/
Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
7,998 Views
•
10:21 min
•
September 05, 2019
DOI:
Summary
Automatically generated
3D single-molecule localization microscopy is utilized to probe the spatial positions and motion trajectories of fluorescently labeled proteins in living bacterial cells. The experimental and data analysis protocol described herein determines the prevalent diffusive behaviors of cytosolic proteins based on pooled single-molecule trajectories.
Read Article
Cite this Article
Rocha, J. M., Gahlmann, A. Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules. J. Vis. Exp. (151), e59387, doi:10.3791/59387 (2019).
Copy