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Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
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September 05, 2019
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3D single-molecule localization microscopy is utilized to probe the spatial positions and motion trajectories of fluorescently labeled proteins in living bacterial cells. The experimental and data analysis protocol described herein determines the prevalent diffusive behaviors of cytosolic proteins based on pooled single-molecule trajectories.
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Rocha, J. M., Gahlmann, A. Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules. J. Vis. Exp. (151), e59387, doi:10.3791/59387 (2019).
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