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Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
Chapters
- 00:04Title
- 00:35Microscope Preparation
- 02:33Y. enterocolitica Culture Preparation
- 03:33Data Acquisition
- 05:54Data Processing
- 06:45Data Post-processing – Overlay of Outlines
- 07:32Results: Determining the Diffusive States of YscQ
- 09:25Conclusion
3D single-molecule localization microscopy is utilized to probe the spatial positions and motion trajectories of fluorescently labeled proteins in living bacterial cells. The experimental and data analysis protocol described herein determines the prevalent diffusive behaviors of cytosolic proteins based on pooled single-molecule trajectories.
