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Traitement d’image protocole pour l’analyse de la Diffusion et la taille de Cluster de récepteurs membranaires par microscopie de Fluorescence
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Traitement d’image protocole pour l’analyse de la Diffusion et la taille de Cluster de récepteurs membranaires par microscopie de Fluorescence
1Department of Macromolecular Structures, Centro Nacional de Biotecnología,Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n,Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología,Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling,Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology,McGill Univ.
Chapters
- 00:04Title
- 01:37Preparation of Biological Samples and Image Acquisition
- 02:57Selection of Images and Creation of Masks
- 05:45Tracking Particles
- 08:09Calculation of the Diffusion Coefficients and Classification of Trajectories
- 09:10Calculation of Cluster Size
- 09:50Results: Analysis of Dynamic Fluorescence Particle Characteristics
- 11:24Conclusion
