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用荧光显微镜分析膜受体扩散和簇簇的图像处理协议
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JoVE 신문 면역학 및 감염병학
Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
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用荧光显微镜分析膜受体扩散和簇簇的图像处理协议

8,467 Views

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12:15 min

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April 09, 2019

DOI:

10.3791/59314-v

DOI:
10.3791/59314-v

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12:15 min
April 09, 2019

•

8 Views
, , , , , ,
1Department of Macromolecular Structures, Centro Nacional de Biotecnología,Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n,Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología,Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling,Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology,McGill Univ.

Summary

Automatically generated

在这里, 我们提出了一个单一粒子跟踪图像分析的协议, 允许定量评估扩散系数, 类型的运动和集群大小的单个粒子检测到的荧光显微镜。

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Sorzano, C. O. S., Martínez-Muñoz, L., Cascio, G., García-Cuesta, E. M., Vargas, J., Mellado, M., Rodriguez Frade, J. M. Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy. J. Vis. Exp. (146), e59314, doi:10.3791/59314 (2019).

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