Here, we present a protocol for single particle tracking image analysis that allows quantitative evaluation of diffusion coefficients, types of motion and cluster sizes of single particles detected by fluorescence microscopy.
Sorzano, C. O. S., Martínez-Muñoz, L., Cascio, G., García-Cuesta, E. M., Vargas, J., Mellado, M., Rodriguez Frade, J. M. Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy. J. Vis. Exp. (146), e59314, doi:10.3791/59314 (2019).