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Measuring Enzymatic Stability by Isothermal Titration Calorimetry
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Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

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08:37 min

March 26, 2019

DOI:

08:37 min
March 26, 2019

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Summary

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The thermal stability of enzyme activity is readily measured by isothermal titration calorimetry (ITC). Most protein stability assays currently used measure protein unfolding, but do not provide information about enzymatic activity. ITC enables direct determination of the effect of enzyme modifications on the stability of enzyme activity.

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