Journal
/
环境科学
/
Measuring Enzymatic Stability by Isothermal Titration Calorimetry
JoVE 杂志
环境科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

12,614 Views

•

08:37 min

•

March 26, 2019

DOI:

10.3791/59302-v

DOI:
10.3791/59302-v

•

08:37 min
March 26, 2019

•

12 Views
, , ,
1Department of Nutrition, Dietetics and Food Science,Brigham Young University, 2Department of Chemistry and Biochemistry,Brigham Young University

Summary

Automatically generated

The thermal stability of enzyme activity is readily measured by isothermal titration calorimetry (ITC). Most protein stability assays currently used measure protein unfolding, but do not provide information about enzymatic activity. ITC enables direct determination of the effect of enzyme modifications on the stability of enzyme activity.

Explore More Videos

Enzyme StabilityIsothermal Titration CalorimetryEnzymatic ReactionsEnergy-efficient CatalystsEnzyme ModificationsEnzyme AssaysSodium Acetate BufferEnzyme SolutionSubstrate Solution

Read Article

Cite this Article

Chan, W. K. D., Mason, M., Hansen, L. D., Kenealey, J. D. Measuring Enzymatic Stability by Isothermal Titration Calorimetry. J. Vis. Exp. (145), e59302, doi:10.3791/59302 (2019).

Download .ris file

Copy

Share Video

.

Copy