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Measuring Enzymatic Stability by Isothermal Titration Calorimetry
章
- 00:04標題
- 00:51Preparing Samples
- 03:00Preparing the ITC (Isothermal Titration Calorimetry) Instrument
- 05:22Setting up the ITCrun and Analyzing Data
- 06:36Results: Enzymatic Stability
- 08:04Conclusion
The thermal stability of enzyme activity is readily measured by isothermal titration calorimetry (ITC). Most protein stability assays currently used measure protein unfolding, but do not provide information about enzymatic activity. ITC enables direct determination of the effect of enzyme modifications on the stability of enzyme activity.
