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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
Chapters
- 00:04Title
- 00:52Isolation of Spleens from Mice
- 01:27Generation of Single Cell Suspensions from Spleens
- 02:39Isolation of CD11c+ DCs from Splenic Single Cell Suspension
- 03:41Magnetic Separation of CD11c+ DCs
- 04:51Adoptive Transfer of High Salt Treated CD11c+ DCs
- 06:56Results: Analysis of the High Salt Treated Antigen-presenting Dendritic Cells
- 08:52Conclusion
Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.
