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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
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JoVE Journal 免疫学と感染
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

DOI:
10.3791/59124-v

•

09:29 min

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March 05, 2019

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, , , ,
1Division of Clinical Pharmacology, Department of Medicine,Vanderbilt University Medical Center

章

  • 00:04標題
  • 00:52Isolation of Spleens from Mice
  • 01:27Generation of Single Cell Suspensions from Spleens
  • 02:39Isolation of CD11c+ DCs from Splenic Single Cell Suspension
  • 03:41Magnetic Separation of CD11c+ DCs
  • 04:51Adoptive Transfer of High Salt Treated CD11c+ DCs
  • 06:56Results: Analysis of the High Salt Treated Antigen-presenting Dendritic Cells
  • 08:52Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

タグ

Dendritic CellsCD11cHypertensionCardiovascular DiseaseAdoptive TransferSpleenIsolationCollagenase DDNaseMACSFlow Cytometry

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