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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
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March 05, 2019
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Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.
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Van Beusecum, J. P., Xiao, L., Barbaro, N. R., Patrick, D. M., Kirabo, A. Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells. J. Vis. Exp. (145), e59124, doi:10.3791/59124 (2019).
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