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Immunology and Infection

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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
 

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Article DOI: 10.3791/59124-v 09:29 min March 5th, 2019
March 5th, 2019

章节

总结概括

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

Tags

Keywords: Dendritic Cells CD11c Hypertension Cardiovascular Disease Adoptive Transfer Spleen Isolation Collagenase D DNase MACS Flow Cytometry
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