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用于向癌细胞输送 sirna 的外质体的制备
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암 연구학
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JoVE 신문 암 연구학
Preparation of Exosomes for siRNA Delivery to Cancer Cells
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用于向癌细胞输送 sirna 的外质体的制备

23,944 Views

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09:59 min

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December 05, 2018

DOI:

10.3791/58814-v

DOI:
10.3791/58814-v

•

09:59 min
December 05, 2018

•

28 Views
, ,
1Institute of Pharmaceutical Science,King’s College London

Summary

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外显子是新一代的药物输送载体。我们建立了一个具有高产量和纯度的非连续分离协议, 用于 sirna 的传递。我们还将荧光标记的非特异性 sirna 封装到外显子体中, 并研究了癌细胞中含有 sirna 的外显子体的细胞吸收情况。

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Cite this Article

Faruqu, F. N., Xu, L., Al-Jamal, K. T. Preparation of Exosomes for siRNA Delivery to Cancer Cells. J. Vis. Exp. (142), e58814, doi:10.3791/58814 (2018).

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