An exosome is a new generation of drug delivery carriers. We established an exosome isolation protocol with high yield and purity for siRNA delivery. We also encapsulated fluorescently labelled non-specific siRNA into exosomes and investigated the cellular uptake of siRNA-loaded exosomes in cancer cells.
Faruqu, F. N., Xu, L., Al-Jamal, K. T. Preparation of Exosomes for siRNA Delivery to Cancer Cells. J. Vis. Exp. (142), e58814, doi:10.3791/58814 (2018).