/
/
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
6,160 Views
•
10:56 min
•
June 22, 2019
DOI:
Summary
Automatically generated
This paper details a protocol for preparing a co-culture of cryptococcal cells and amoebae that is studied using still, fluorescent images and high-resolution transmission electron microscope images. Illustrated here is how quantitative data can complement such qualitative information.
Explore More Videos
Microscopic TechniquesFluorescence ReadingCryptococcus-Amoeba InteractionsPhagocytosisPathogenic FungusCryptococcus NeoformansEnvironmental PredatorAmoebaConfocal Laser-scanning MicroscopyTransmission Electron MicroscopyYPD AgarYNB BrothGlucoseAmoeba CellsPeptone Yeast Extract Glucose BrothTrypan BlueFluorescein IsothiocyanatePBSGlutaraldehydeCo-culture
Read Article
Cite this Article
Madu, U. L., Sebolai, O. M. Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions. J. Vis. Exp. (148), e58698, doi:10.3791/58698 (2019).
Copy