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Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
JoVE Journal
Ormancılık ve botanik
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JoVE Journal Ormancılık ve botanik
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

DOI:
10.3791/58698-v

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10:56 min

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June 22, 2019

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,
1Department of Microbial, Biochemical and Food Biotechnology,University of the Free State

'Bölümler'

  • 00:00'Başlık'
  • 00:40Cultivation and Standardization of Fungal Cells
  • 01:54Cultivation and Standardization of Amoeba Cells
  • 02:34Fluorescence Staining of Cells to Study Phagocytosis
  • 04:50Usage of Transmission Electron Microscopy to Study Phagocytosis
  • 07:02'Sonuçlar'
  • 09:31Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This paper details a protocol for preparing a co-culture of cryptococcal cells and amoebae that is studied using still, fluorescent images and high-resolution transmission electron microscope images. Illustrated here is how quantitative data can complement such qualitative information.

'Etiketler'

Microscopic TechniquesFluorescence ReadingCryptococcus-Amoeba InteractionsPhagocytosisPathogenic FungusCryptococcus NeoformansEnvironmental PredatorAmoebaConfocal Laser-scanning MicroscopyTransmission Electron MicroscopyYPD AgarYNB BrothGlucoseAmoeba CellsPeptone Yeast Extract Glucose BrothTrypan BlueFluorescein IsothiocyanatePBSGlutaraldehydeCo-culture

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