Journal
/
Immunology and Infection
/
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

DOI:
10.3791/58698-v

•

10:56 min

•

June 22, 2019

•

,
1Department of Microbial, Biochemical and Food Biotechnology,University of the Free State

Chapters

  • 00:00Title
  • 00:40Cultivation and Standardization of Fungal Cells
  • 01:54Cultivation and Standardization of Amoeba Cells
  • 02:34Fluorescence Staining of Cells to Study Phagocytosis
  • 04:50Usage of Transmission Electron Microscopy to Study Phagocytosis
  • 07:02Results
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

This paper details a protocol for preparing a co-culture of cryptococcal cells and amoebae that is studied using still, fluorescent images and high-resolution transmission electron microscope images. Illustrated here is how quantitative data can complement such qualitative information.

Tags

Keywords: Microscopic TechniquesFluorescence ReadingCryptococcus-Amoeba InteractionsPhagocytosisPathogenic FungusCryptococcus NeoformansEnvironmental PredatorAmoebaConfocal Laser-scanning MicroscopyTransmission Electron MicroscopyYPD AgarYNB BrothGlucoseAmoeba CellsPeptone Yeast Extract Glucose BrothTrypan BlueFluorescein IsothiocyanatePBSGlutaraldehydeCo-culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58698?article_type=v

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
check_url/58698?article_type=v

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
check_url/58698?article_type=v

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
check_url/58698?article_type=v

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
check_url/58698?article_type=v

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
check_url/58698?article_type=v

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
check_url/58698?article_type=v

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
check_url/58698?article_type=v

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants
check_url/58698?article_type=v

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
check_url/58698?article_type=v

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Read Article