Journal
/
발생학
/
Fluorimetric Techniques for the Assessment of Sperm Membranes
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

12,863 Views

•

08:58 min

•

November 28, 2018

DOI:

10.3791/58622-v

DOI:
10.3791/58622-v

•

08:58 min
November 28, 2018

•

27 Views
,
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem

Summary

Automatically generated

Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.

Explore More Videos

Fluorimetric TechniquesSperm MembranesFluorescent ProbesFluorescence MicroscopyFlow CytometrySperm Fertilization PotentialSperm Plasma MembraneAcrosome MembraneMitochondrial Membrane PotentialNKM BufferDAPIFITC-PSAJC-1PIEpifluorescence Microscopy

Read Article

Cite this Article

Komsky-Elbaz, A., Roth, Z. Fluorimetric Techniques for the Assessment of Sperm Membranes. J. Vis. Exp. (141), e58622, doi:10.3791/58622 (2018).

Download .ris file

Copy

Share Video

.

Copy