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Fluorimetric Techniques for the Assessment of Sperm Membranes
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

12,848 Views

08:58 min

November 28, 2018

DOI:

08:58 min
November 28, 2018

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Summary

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Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.

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