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Fluorimetric Techniques for the Assessment of Sperm Membranes
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JoVE Journal Gelişim Biyolojisi
Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

12,848 Views

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08:58 min

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November 28, 2018

DOI:

10.3791/58622-v

DOI:
10.3791/58622-v

•

08:58 min
November 28, 2018

•

27 Views
,
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem

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Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.

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Komsky-Elbaz, A., Roth, Z. Fluorimetric Techniques for the Assessment of Sperm Membranes. J. Vis. Exp. (141), e58622, doi:10.3791/58622 (2018).

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