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Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
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Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
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November 29, 2018
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We provide a method for the generation, cultivation and systematic analysis of organotypic slices derived from murine lung tumors. We also describe how to optimize for slice thickness, and how to select drug concentrations to treat tumor slices.
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Nagaraj, A. S., Bao, J., Hemmes, A., Machado, M., Närhi, K., Verschuren, E. W. Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J. Vis. Exp. (141), e58569, doi:10.3791/58569 (2018).
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