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Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

13,625 Views

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11:00 min

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November 29, 2018

DOI:

10.3791/58569-v

DOI:
10.3791/58569-v

•

11:00 min
November 29, 2018

•

5 Views
, , , , ,
1Institute for Molecular Medicine Finland (FIMM), HiLIFE,University of Helsinki

Summary

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We provide a method for the generation, cultivation and systematic analysis of organotypic slices derived from murine lung tumors. We also describe how to optimize for slice thickness, and how to select drug concentrations to treat tumor slices.

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Nagaraj, A. S., Bao, J., Hemmes, A., Machado, M., Närhi, K., Verschuren, E. W. Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J. Vis. Exp. (141), e58569, doi:10.3791/58569 (2018).

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