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Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

13,609 Views

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11:00 min

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November 29, 2018

DOI:

10.3791/58569-v

DOI:
10.3791/58569-v

•

11:00 min
November 29, 2018

•

5 Views
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1Institute for Molecular Medicine Finland (FIMM), HiLIFE,University of Helsinki

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We provide a method for the generation, cultivation and systematic analysis of organotypic slices derived from murine lung tumors. We also describe how to optimize for slice thickness, and how to select drug concentrations to treat tumor slices.

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Nagaraj, A. S., Bao, J., Hemmes, A., Machado, M., Närhi, K., Verschuren, E. W. Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J. Vis. Exp. (141), e58569, doi:10.3791/58569 (2018).

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