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肾小球的分离及肾小球细胞表面蛋白的活体标记

JoVE 신문
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JoVE 신문 생화학

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

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肾小球的分离及肾小球细胞表面蛋白的活体标记

DOI:

10.3791/58542-v

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09:12 min

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January 18, 2019

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Eva Königshausen, Sebastian A. Potthoff, Raphael Haase, Catherine Meyer-Schwesinger, Ernest Kaufmann, L. Christian Rump, Johannes Stegbauer, Lorenz Sellin, Ivo Quack, Magdalena Woznowski

¹Department of Nephrology, Medical Faculty,Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology,University Medical Center Hamburg-Eppendorf

Chapters

00:04Title
00:47Surgical Procedure
03:00In Vivo Biotinylation and Glomeruli Isolation
05:29Glomeruli Washing and Quantification
06:25Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins
08:31Conclusion

Summary

자동 번역

在这里, 我们提出了一个协议的小鼠体内标记肾小球细胞表面蛋白与生物素.该协议包含有关如何灌注小鼠肾脏, 分离肾小球, 并执行内源性免疫沉淀的感兴趣的蛋白质的信息。

Tags

Glomeruli Isolation In Vivo Labeling Glomerular Cell Surface Proteins Proteinuric Kidney Disease Cell Surface Protein Trafficking Organ Perfusion Kidney Isolation Aortic Perfusion Renal Vein PBS Perfusion

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