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Cancer Research

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JoVE Journal Cancer Research
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
 

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

Article DOI: 10.3791/58492-v • 12:16 min • October 16th, 2018
October 16th, 2018 •
1, 1, 1, 1,2, 1
1Manchester Cancer Research Centre Building, Department Cancer Sciences, University of Manchester, 2Celyad

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Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.

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Syngeneic Mouse B-cell Lymphoma Model Pre-clinical Evaluation CD19 CAR T Cells Genetic Modification Combination Therapy Lympho Replete Lympho Depleted Endogenous Immune Cells Disease Progression Therapeutic CAR T Cells Solid Tumor Diagnosis Lessons Learned Translation To Other Targets Media Preparation Cell Seeding CD19 Chimeric Antigen Receptor (CAR) T-cell Therapy Second Generation CARs Acute Lymphoblastic Leukemia (ALL) Non-Hodgkin Lymphoma (NHL) Hematological Malignancies Complete Response Rates
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