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A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
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A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
Chapitres
- 00:04Titre
- 00:55Days 1 & 2: Preparation and Transfection of Plat-E Retroviral Packaging Cell Line
- 02:11Day 3: Preparation of Virus-Containing Supernatant for Transduction and Isolation and In Vitro Activation of Mouse Splenic T Cells
- 04:27Day 4: Transduction of Mouse T Cells
- 06:43Day 5: Round 2 of Transduction
- 08:33In Vitro Validation of CAR T Cell Activity
- 09:53Results: Pre-Clinical Evaluation of CD19 CAR T Cells
- 11:32Conclusion
Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.
