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A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
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A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

14,083 Views

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12:16 min

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October 16, 2018

DOI:

10.3791/58492-v

DOI:
10.3791/58492-v

•

12:16 min
October 16, 2018

•

14 Views
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1Manchester Cancer Research Centre Building, Department Cancer Sciences,University of Manchester, 2Celyad

Summary

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Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.

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CD19 CAR T CellsSyngeneic Mouse ModelB-cell LymphomaPre-clinical EvaluationLymphodepletionAnti-cancer ActivityRetroviral TransductionEx Vivo Validation

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Cite this Article

Kueberuwa, G., Zheng, W., Kalaitsidou, M., Gilham, D. E., Hawkins, R. E. A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells. J. Vis. Exp. (140), e58492, doi:10.3791/58492 (2018).

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