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Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
Chapters
- 00:04Title
- 00:54Isolating Mitochondria from Dairy Cow Liver
- 02:46Measuring Mitochondrial Oxygen Consumption
- 04:33Measuring Mitochondrial Membrane Potential (MMP) and Proton Motive Force (PMF)
- 06:42Results: Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics
- 07:45Conclusion
Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.
