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Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
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生物化学
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JoVE 杂志 生物化学
Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

DOI:
10.3791/58387-v

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08:29 min

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November 30, 2018

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, , ,
1Veterinary Medicine Teaching and Research Center,University of California Davis, 2Archer Daniels Midland, 3Department of Molecular Biosciences,University of California Davis

Chapters

  • 00:04Title
  • 00:54Isolating Mitochondria from Dairy Cow Liver
  • 02:46Measuring Mitochondrial Oxygen Consumption
  • 04:33Measuring Mitochondrial Membrane Potential (MMP) and Proton Motive Force (PMF)
  • 06:42Results: Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics
  • 07:45Conclusion

Summary

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Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.

Tags

Keywords: Mitochondrial Oxygen ConsumptionProton Leak KineticsMitochondrial RespirationDairy CattleEnergeticsMitochondrial IsolationLiver SampleHomogenizationCentrifugationProtein ConcentrationOxygen Consumption AssayRespiration ChamberOxygraph System

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