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JoVE Journal Biochemistry
Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
 

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

Article DOI: 10.3791/58387-v • 08:29 min • November 30th, 2018
November 30th, 2018 •
1, 2, 1, 3
1Veterinary Medicine Teaching and Research Center, University of California Davis, 2Archer Daniels Midland, 3Department of Molecular Biosciences, University of California Davis

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Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.

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Liver Mitochondrial Oxygen Consumption Proton Leak Kinetics Mitochondrial Respiration Holstein Dairy Cattle Energetics Field Mitochondrial Health Energetic Efficiency Energy Usage Oxygen Consumption Intracellular Metabolic Processes Feed Efficiency Video Demonstration Mitochondrial Isolation Red Blood Cells Tissue Mincing Homogenization Centrifugation
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