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Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
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October 08, 2018
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Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.
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Luukinen, H., Hammarén, M. M., Vanha-aho, L., Parikka, M. Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish. J. Vis. Exp. (140), e58299, doi:10.3791/58299 (2018).
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