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Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
Chapters
- 00:04Title
- 00:37Culturing of Mycobacterium marinum and Preparation of Bacterial Solution for Infecting Adult Zebrafish
- 02:10Experimental M. marinum Infection with Intraperitoneal Injection
- 03:32Collection of Internal Organs
- 04:36Results: Mycobacterium marinum Infection Modeling
- 06:02Conclusion
Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.
