Journal
/
신경과학
/
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

17,790 Views

•

05:00 min

•

September 05, 2018

DOI:

10.3791/58288-v

DOI:
10.3791/58288-v

•

05:00 min
September 05, 2018

•

31 Views
, , , , , ,
1Institute of Life Science,Nanchang University, 2Queen Mary School, Medical Department,Nanchang University, 3School of Life Science,Nanchang University

Summary

Automatically generated

Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.

Explore More Videos

SectioningCutting And Floating MethodFume HoodAcrylic BoardsChloroformSection ChannelWater BathHeating ElementDigital ThermostatMicrotomeNeutral Silicone SealantParaffin SectioningTemperature Detection SwitchParaffin BlockCassette ClampSection ThicknessFloating SectionsMicroscope Slide

Read Article

Cite this Article

Qin, C., Bai, Y., Zeng, Z., Wang, L., Luo, Z., Wang, S., Zou, S. The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning. J. Vis. Exp. (139), e58288, doi:10.3791/58288 (2018).

Download .ris file

Copy

Share Video

.

Copy