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The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
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JoVE Journal 神経科学
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

17,790 Views

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05:00 min

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September 05, 2018

DOI:

10.3791/58288-v

DOI:
10.3791/58288-v

•

05:00 min
September 05, 2018

•

31 Views
, , , , , ,
1Institute of Life Science,Nanchang University, 2Queen Mary School, Medical Department,Nanchang University, 3School of Life Science,Nanchang University

概要

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Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.

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Cite this Article

Qin, C., Bai, Y., Zeng, Z., Wang, L., Luo, Z., Wang, S., Zou, S. The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning. J. Vis. Exp. (139), e58288, doi:10.3791/58288 (2018).

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