Journal
/
神经科学
/
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

17,790 Views

•

05:00 min

•

September 05, 2018

DOI:

10.3791/58288-v

DOI:
10.3791/58288-v

•

05:00 min
September 05, 2018

•

31 Views
, , , , , ,
1Institute of Life Science,Nanchang University, 2Queen Mary School, Medical Department,Nanchang University, 3School of Life Science,Nanchang University

Summary

Automatically generated

Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.

Explore More Videos

SectioningCutting And Floating MethodFume HoodAcrylic BoardsChloroformSection ChannelWater BathHeating ElementDigital ThermostatMicrotomeNeutral Silicone SealantParaffin SectioningTemperature Detection SwitchParaffin BlockCassette ClampSection ThicknessFloating SectionsMicroscope Slide

Read Article

Cite this Article

Qin, C., Bai, Y., Zeng, Z., Wang, L., Luo, Z., Wang, S., Zou, S. The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning. J. Vis. Exp. (139), e58288, doi:10.3791/58288 (2018).

Download .ris file

Copy

Share Video

.

Copy