/
/
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Chapters
- 00:04Title
- 00:48General Lymantria dispar (LD652) Cell and Virus Culture
- 01:33General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
- 02:43Luciferase Assay
- 04:06Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
- 05:04Conclusion
Here, we present the protocols to identify 1) virus-encoded immunomodulators that promote arbovirus replication and 2) eukaryotic host factors that restrict arbovirus replication. These fluorescence- and luminescence-based methods allow researchers to rapidly obtain quantitative readouts of arbovirus replication in simplistic assays with low signal-to-noise ratios.
