Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Arbovirus Infections As Screening Tools for the…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
 

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Article DOI: 10.3791/58244-v • 06:02 min • September 13th, 2018
September 13th, 2018 •
1, 1, 1
1Department of Microbiology, University of Texas Southwestern Medical Center

Capitoli

Riepilogo
Automatic Translation

Here, we present the protocols to identify 1) virus-encoded immunomodulators that promote arbovirus replication and 2) eukaryotic host factors that restrict arbovirus replication. These fluorescence- and luminescence-based methods allow researchers to rapidly obtain quantitative readouts of arbovirus replication in simplistic assays with low signal-to-noise ratios.

Tags

Arbovirus Infections Screening Tools Viral Immunomodulators Host Antiviral Factors Viral Immunology Host Factors Arbovirus Replication Virus-encoded Immune Evasion Proteins Luminescence Assay Fluorescent Space Assay Lepidopteran Insect Cells Background Replication LD652 Cells Tissue Culture-treated Dishes Cell Monolayer Growth Media 24-well Plate VSV Luciferase Vaccinia Virus MOI
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter