需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Chapters
- 00:04Title
- 00:48General Lymantria dispar (LD652) Cell and Virus Culture
- 01:33General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
- 02:43Luciferase Assay
- 04:06Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
- 05:04Conclusion
Here, we present the protocols to identify 1) virus-encoded immunomodulators that promote arbovirus replication and 2) eukaryotic host factors that restrict arbovirus replication. These fluorescence- and luminescence-based methods allow researchers to rapidly obtain quantitative readouts of arbovirus replication in simplistic assays with low signal-to-noise ratios.
