Journal
/
화학
/
Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

DOI:
10.3791/58141-v

•

08:26 min

•

August 31, 2018

•

,
1Department of Physics and Optical Science, Center for Biomedical Engineering & Science,University of North Carolina

Chapters

  • 00:04Title
  • 00:22Synthesis of BSA-Au(III) Complex
  • 01:14Synthesis of BSA-Cu(II)-Au(III)
  • 02:29Synthesis of BSA-Ni(II)-Au(III)
  • 03:26Synthesis of [Cys34-capped-BSA]-Au(III)
  • 04:45Synthesis of [all-thiol-capped-BSA]-Au(III)
  • 06:57Results: Conformational Change-induced Fluorescence of Bovine Serum Albumin-gold Complexes
  • 08:06Conclusion

Summary

자동 번역

자동 번역

The protocols for studying the binding of gold cations (Au(III)) to various conformations of bovine serum albumin (BSA) as well as for characterizing the conformational dependent unique BSA-Au fluorescence are presented.

Tags

Bovine Serum AlbuminGold(III)Luminophore FormationMetal BindingProtein ComplexesNanoscienceChloroauric AcidCopper ChlorideNickel ChlorideN-EthylmaleimidePH AdjustmentSpectroscopy

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Microwave-assisted Functionalization of Poly(ethylene glycol) and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Read Article