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Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
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JoVE Journal Chemistry
Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

DOI:
10.3791/58141-v

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08:26 min

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August 31, 2018

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,
1Department of Physics and Optical Science, Center for Biomedical Engineering & Science,University of North Carolina

Chapters

  • 00:04Title
  • 00:22Synthesis of BSA-Au(III) Complex
  • 01:14Synthesis of BSA-Cu(II)-Au(III)
  • 02:29Synthesis of BSA-Ni(II)-Au(III)
  • 03:26Synthesis of [Cys34-capped-BSA]-Au(III)
  • 04:45Synthesis of [all-thiol-capped-BSA]-Au(III)
  • 06:57Results: Conformational Change-induced Fluorescence of Bovine Serum Albumin-gold Complexes
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

The protocols for studying the binding of gold cations (Au(III)) to various conformations of bovine serum albumin (BSA) as well as for characterizing the conformational dependent unique BSA-Au fluorescence are presented.

Tags

Bovine Serum AlbuminGold(III)Luminophore FormationMetal BindingProtein ComplexesNanoscienceChloroauric AcidCopper ChlorideNickel ChlorideN-EthylmaleimidePH AdjustmentSpectroscopy

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