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Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
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Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

DOI:
10.3791/58141-v

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08:26 min

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August 31, 2018

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,
1Department of Physics and Optical Science, Center for Biomedical Engineering & Science,University of North Carolina

Chapitres

  • 00:04Titre
  • 00:22Synthesis of BSA-Au(III) Complex
  • 01:14Synthesis of BSA-Cu(II)-Au(III)
  • 02:29Synthesis of BSA-Ni(II)-Au(III)
  • 03:26Synthesis of [Cys34-capped-BSA]-Au(III)
  • 04:45Synthesis of [all-thiol-capped-BSA]-Au(III)
  • 06:57Results: Conformational Change-induced Fluorescence of Bovine Serum Albumin-gold Complexes
  • 08:06Conclusion

Summary

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The protocols for studying the binding of gold cations (Au(III)) to various conformations of bovine serum albumin (BSA) as well as for characterizing the conformational dependent unique BSA-Au fluorescence are presented.

Tags

Bovine Serum AlbuminGold(III)Luminophore FormationMetal BindingProtein ComplexesNanoscienceChloroauric AcidCopper ChlorideNickel ChlorideN-EthylmaleimidePH AdjustmentSpectroscopy

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