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分光光度法测定蓝藻Synechocystis中的蛋白含量
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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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分光光度法测定蓝藻Synechocystis中的蛋白含量

DOI:
10.3791/58076-v

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08:07 min

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September 11, 2018

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, , ,
1Department of Adaptive Biotechnologies, Global Change Research Institute,Czech Academy of Sciences, 2Department of Plant Physiology, Faculty of Science,Masaryk University, 3Laboratory of Intracellular Regulation, Institute of Plant Physiology,Russian Academy of Sciences

Chapters

  • 00:00Title
  • 01:10Samples Preparation
  • 02:34Cells Homogenization and Pigment Extraction
  • 03:47Phycobiliproteins Quantification
  • 04:47Representative Results
  • 07:30Conclusion

Summary

자동 번역

자동 번역

在这里, 我们提出了一个协议, 以定量地确定蛋白的内容在蓝藻Synechocystis使用分光光度法。提取过程也成功地应用于其他蓝藻和藻类菌株;然而, 由于颜料吸收谱的变化, 有必要分别测试每种菌株的分光光度方程。

Tags

Spectrophotometric DeterminationPhycobiliproteinsCyanobacteriumSynechocystisPhycocyaninAllophycocyaninPigment-protein ComplexesLight-harvesting AntennaeCyanobacteria ProductivityExtractionFreeze-dryingHomogenization

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