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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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JoVE Revista Bioquímica
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

14,783 Views

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08:07 min

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September 11, 2018

DOI:

10.3791/58076-v

DOI:
10.3791/58076-v

•

08:07 min
September 11, 2018

•

17 Views
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1Department of Adaptive Biotechnologies, Global Change Research Institute,Czech Academy of Sciences, 2Department of Plant Physiology, Faculty of Science,Masaryk University, 3Laboratory of Intracellular Regulation, Institute of Plant Physiology,Russian Academy of Sciences

Summary

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Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.

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Zavřel, T., Chmelík, D., Sinetova, M. A., Červený, J. Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis. J. Vis. Exp. (139), e58076, doi:10.3791/58076 (2018).

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