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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Capítulos
- 00:00Título
- 01:10Samples Preparation
- 02:34Cells Homogenization and Pigment Extraction
- 03:47Phycobiliproteins Quantification
- 04:47Representative Results
- 07:30Conclusion
Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.
