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A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
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A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
Chapters
- 00:04Title
- 00:54Preparation of Target Cells for Cell-to-Cell Virus Transmission
- 02:11Preparation of Donor Cells
- 03:56Co-culture
- 05:06Fluorescence-activated Cell Sorting
- 05:35Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
- 06:49Conclusion
We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.
