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A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

6,225 Views

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07:22 min

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August 14, 2018

DOI:

10.3791/58074-v

DOI:
10.3791/58074-v

•

07:22 min
August 14, 2018

•

1 Views
, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Biyoloji Bölümü,New Jersey City University

Özet

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We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.

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Cite this Article

Esposito, A. M., Soare, A. Y., Patel, F., Satija, N., Chen, B. K., Swartz, T. H. A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (138), e58074, doi:10.3791/58074 (2018).

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