Journal
/
Immunologie und Infektion
/
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

6,225 Views

•

07:22 min

•

August 14, 2018

DOI:

10.3791/58074-v

DOI:
10.3791/58074-v

•

07:22 min
August 14, 2018

•

1 Views
, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Summary

Automatically generated

We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.

Explore More Videos

Cre-LoxViral Membrane FusionHIV-1Drug DiscoveryHigh-throughput ScreeningHIV Fusion InhibitorsBiosafetyJurkat RG Reporter CellsPuromycinCell-to-cell Virus TransmissionGag-iCre DNAElectroporationFicoll

Read Article

Cite this Article

Esposito, A. M., Soare, A. Y., Patel, F., Satija, N., Chen, B. K., Swartz, T. H. A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (138), e58074, doi:10.3791/58074 (2018).

Download .ris file

Copy

Share Video

.

Copy