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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

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08:26 min

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June 25, 2018

DOI:

10.3791/57979-v

DOI:
10.3791/57979-v

•

08:26 min
June 25, 2018

•

1 Views
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1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University, 2College of Life Science,Nanjing Agricultural University

Summary

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We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

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N-glycansRaphanus SativusPNGase H+Plant GlycoscienceProtein IsolationTCA PrecipitationN-glycan ReleaseSolid-phase ExtractionFluorescence Derivatization

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Cite this Article

Du, Y., Zheng, S., Liu, L., Voglmeir, J., Yedid, G. Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+. J. Vis. Exp. (136), e57979, doi:10.3791/57979 (2018).

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