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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
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Biochimica
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JoVE Journal Biochimica
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

DOI:
10.3791/57979-v

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08:26 min

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June 25, 2018

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, , , ,
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University, 2College of Life Science,Nanjing Agricultural University

Capitoli

  • 00:04Titolo
  • 00:41Isolation of Protein from Radish and Preparation of N-Glycans
  • 02:35Purification and Fluorescence Derivatization of N-Glycans
  • 04:47HILIC-UPLC profiling of N-Glycans and MALDI-TOF MS Analysis
  • 06:24Results: N-glycan Analysis
  • 06:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

Tags

N-glycansRaphanus SativusPNGase H+Plant GlycoscienceProtein IsolationTCA PrecipitationN-glycan ReleaseSolid-phase ExtractionFluorescence Derivatization

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