Journal
/
生物化学
/
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

6,497 Views

•

08:26 min

•

June 25, 2018

DOI:

10.3791/57979-v

DOI:
10.3791/57979-v

•

08:26 min
June 25, 2018

•

1 Views
, , , ,
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University, 2College of Life Science,Nanjing Agricultural University

Summary

Automatically generated

We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

Explore More Videos

N-glycansRaphanus SativusPNGase H+Plant GlycoscienceProtein IsolationTCA PrecipitationN-glycan ReleaseSolid-phase ExtractionFluorescence Derivatization

Read Article

Cite this Article

Du, Y., Zheng, S., Liu, L., Voglmeir, J., Yedid, G. Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+. J. Vis. Exp. (136), e57979, doi:10.3791/57979 (2018).

Download .ris file

Copy

Share Video

.

Copy