Journal
/
면역학 및 감염병학
/
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

7,957 Views

•

09:44 min

•

June 13, 2018

DOI:

10.3791/57651-v

DOI:
10.3791/57651-v

•

09:44 min
June 13, 2018

•

4 Views
, , ,
1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine,Saarland University

Summary

Automatically generated

Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.

Explore More Videos

Light-sheet MicroscopyThree-dimensional VisualizationHuman Immune CellsImmunologyCell BehaviorPhysiologically Relevant ContextCollagenFluorescent LabelingCell CultureCapillaryImaging

Read Article

Cite this Article

Schoppmeyer, R., Zhao, R., Hoth, M., Qu, B. Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells. J. Vis. Exp. (136), e57651, doi:10.3791/57651 (2018).

Download .ris file

Copy

Share Video

.

Copy